Department Of Biotechnology - Maharashtra

40479252 tender for supply, installation, testing, commissioning, qualification and validation of c gmp compliant mammalian cell line repository on turnkey basis at nccs jidnyasa building, kothrud, pune 411038...

National Centre For Cell Science - Maharashtra

40464122 tender for supply, installation, testing and commissioning and validation of c gmp compliant mammalian cell line repository on turnkey basis at nccs jidnyasa building, kothrud, pune...

Department Of Atomic Energy - Maharashtra

39921227 bids are invited for pha m, lyophilized powder, for mammalian cell culture, salt free (q3) , cytochalasin b(from drechslera dematioidea) , powder, dissolve in dmso (q3) , pha p, lyophilized powder; cell culture for mammalian (q3) , 6 tg (q3) total quantity : 24...

Department Of Atomic Energy - Maharashtra

39448284 bids are invited for sirt1 1f3 mouse mab , tri methyl histone h3 lys27 rabbit monoclonal antibody , tri methyl histone h3 lys9 rabbit monoclonal antibody , acetyl histone h3 lys9 c5b11 rabbit monoclonal antibody , acetyl histone h3 lys27 d5e4 xpr rabbit monoclonal antibody , acetyl histone h3 lys18 d8z5h rabbit mab , phospho histone h2a x ser139 20e3 rabbit mab , sirt6 recombinant rabbit monoclonal antibody , sirt7 mouse monoclonal antibody , tri methyl histone h3 lys4 c42d8 rabbit mab , goat anti mouse igg alexa fluor 647 , goat anti rabbit igg alexa fluor 647 , goat anti rabbit igg alexa fluor 488 , goat anti mouse igg alexa fluor 488 , cytotoxicity kit for mammalian cells mse total quantity : 16...

Maharashtra University Of Health Science - Maharashtra

38771650 tender for supply of reagents and consumables dna extraction kits gene direct pcr kit (for mammalian cell lines, bacteria & yeast) filter tips (1 200 μl) filter tips (1000μl) filter tips (0.1 10μl) 10x teb depc treated dnase/rnase free water molecular grade water del (hpyf31) restriction enzyme edta blood collection tubes trizol ethanol molecular grade primers isopropanol chloroform xylene pcr 8 tubes strip pcr 96 well plate compatible with abi 75001 0.2 ml pcr tubes 1.5 ml pcr tubes 0.5 ml pcr tubes...

Indian Army - Maharashtra

36382240 bids are invited for pathhology consumables mitomycin c fromstreptomyces caespitosus 2mg powder form vial suitable for mammalian cell culture , peripheral blood medium for in vitro cytogenetic analysis fully supplemented culture system containing rpmi medium with l gtutamine fbs pham penicillin streptomycin and sodium bicarbonate , pb medium for in vitro cytogenetic analysis fully supplemented culture system containing rpmi medium with l gtutamine fbs penicillin streptomycin and sodium bicarbonate without mitogen pha m total quantity : 48...

Directorate Of Purchase And Stores - Maharashtra

35628452 supply of mouse peroxiredoxin mouse peroxiredoxin as per annexure attached . , mouse peroxiredoxin 5 lentiviral activation particles, pack of 200 microlitres of transduction ready, high titer crispr / dcas9 lentiviral activation particle, based on synergistic activation mediator ( sam ) transcription activation system consisting ofdeactivated cas9 ( dcas9 ) nuclease ( d10a and n863a ) fused to the transactivation domain vp64, an ms2 p65 hsf1 fusion protein and a target specific 20 nucleotide guide rna, provides robust recruitment of transcription factors for highly efficient gene activation of prdx5, must be replication incompetent and are designed to self inactivate after transduction and integration into genomic dna of target cells [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] , mouse peroxiredoxin 5 double nickase plasmid, pack of 20 micrograms, suitable for up to 20 transfections, consists of a pair of plasmids each encoding a d10a mutated cas9 nuclease and a target specific 20 nt guide rna ( grna ) designed to knockout gene expression with greater specificity than its crispr / cas9 ko counterpart, paired grna sequences are offset by approximately 20 bp to allow for specific cas9 mediated double nicking of the genomic dna, which mimics a dsb; antibiotic and gfp marker on plasmids for selection and visual confirmation [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] , mouse peroxiredoxin 5 lentiviral inactivation particles, pack of 200 ?l viral stock containing 1000000 infectious units of virus ( ifu ) , provided as transduction ready viral particles for gene silencing, specific for mouse prdx5, contain three to five expression constructs each encoding target specific 19 25 nt ( plus hairpin ) shrna designed to knockdown gene expression, puromycin or similar antibiotic selection marker, replication incompetent and are designed to self inactivate after transduction and integration of shrna constructs into genomic dna of target cells [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] , 1, 5 dimethyl 1, 5 diazaundecamethylene polymethobromide, pack of 1 ml, concentration of 10 mg / ml, infection reagent used to introduce retroviral vectors into mammalian cells, meant to be used for cell culture by manufacturer [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] , negative control lentiviral activation particles, pack of 200 microlites of transduction ready, high titer crispr / dcas9 lentiviral activation particle, based on synergistic activation mediator ( sam ) transcription activation system consisting ofdeactivated cas9 ( dcas9 ) nuclease ( d10a and n863a ) fused to the transactivation domain vp64, an ms2 p65 hsf1 fusion protein and a non specific 20 nucleotide guide rna, sam complex does not recognize or bind any specific sequence within the genomic dna, and does not activate transcription of any specific gene, must be replication incompetent and are designed to self inactivate after transduction [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] , control lentiviral particles with gfp coding construct to express in mammalian cells, pack of 200 microlites of transduction ready viral particles sufficient for 10 or more transductions, stable expression of gfp after transduction, meant to measure transduction efficiency in cell type, replication incompetent and are designed to self inactivate after transduction and integration of gfp control constructs into genomic dna of target cells [ vendor should provide catalogue number / specification sheet of product that vendor wishes to supply to allow us to cross check specifications ] ...

Directorate Of Purchase And Stores - Maharashtra

35060914 tender for tc treated mammalian cell culture dish, gamma supply of tc treated mammalian cell culture dish, gamma sterilized as per technical specification given in attachment , supply of :100 mm tc treated mammalian cell culture dish, gamma sterilized. 1 ) material: polystyene 2 ) bottom: flat 3 ) shape: round 4 ) lid: lid with airvent 5 ) size: 100mm 6 ) sterilization: gamma sterilization 7 ) provision of stacking: yes , 150 mm tc treated mammalian cell culture dish, gamma sterilized. 1 ) material: polystyene 2 ) bottom: flat 3 ) shape: round 4 ) lid: lid with airvent 5 ) size: 100mm 6 ) sterilization: gamma sterilization 7 ) provision of stacking: yes , 60 mm tc treated mammalian cell culture dish, gamma sterilized. 1 ) material: polystyene 2 ) bottom: flat 3 ) shape: round 4 ) lid: lid with airvent 5 ) size: 100mm 6 ) sterilization: gamma sterilization 7 ) provision of stacking: yes...

Directorate Of Purchase And Stores - Maharashtra

34958068 supply of mouse ptgs2 ( nm_011198 ) supply of mouse ptgs2 ( nm_011198 ) , mouse ptgs2 ( nm_011198 ) 10 microgram ( 10ug ) orf clone, gene: ptgs2 species: house mouse accession no.: nm_011198.4 mus musculus prostaglandin endoperoxide synthase 2 ( ptgs2 ) , mrna vector pcdna3.1+ / c ( k ) dyk genscript #cat. no. omu19313 , ptgs2 ( nm_011198 ) mouse untagged clone, 10ug, origene cat no: #mc219616 type: mouse untagged clone, tag: tag free, symbol: ptgs2 vector: pcmv6 entry e. coli selection: kanamycin ( 25 ug / ml ) mammalian cell selection: neomycin restriction sites: sgfi mlui, accn: nm_011198 insert size: 1815 bp, uniprot id: q05769 , silencer pre designed sirna, 5nmol assay id: 151322, gene: ptgs2, species: mouse thermo fischer catalogue no: am16708 , silencer pre designed sirna, 5nmol assay id: 151324, gene: ptgs2, species: mouse thermo fischer catalogue no: am16708...

Central Purchase Organisation - Maharashtra

34935810 supply of ultra leaf purified anti mouse cd279 ( pd 1 ) antibody supply and safe delivery ofultra leaf purified anti mouse cd279 ( pd 1 ) antibody as per annexure , supply and safe delivery ofultra leaf purified anti mouse cd279 ( pd 1 ) antibody antibody clone: rmp1 14 isotype: rat igg2a, ? reactivity: mouse antibody type: monoclonal host species: rat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , ultra leaf purified anti mouse cd152 antibody antibody clone: uc10 4b9 isotype: armenian hamster igg reactivity: mouse antibody type: monoclonal host species: armenian hamster formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , pe / cyanine5 armenian hamster igg isotype ctrl antibody reactivity: mouse antibody type: monoclonal host species: armenian hamster formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , purified anti mouse cd366 ( tim 3 ) antibody antibody clone: b8.2c12 isotype: rat igg1, ? reactivity: mouse antibody type: monoclonal host species: rat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 500 ?g / vial , ultra leaf purified anti mouse cd223 ( lag 3 ) recombinant antibody antibody clone: qa20a93 isotype: mouse igg1, ? reactivity: mouse antibody type: monoclonal host species: mouse formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , ultra leaf purified anti mouse tigit ( vstm3 ) antibody antibody clone: 4d4 / mtigit isotype: armenian hamster igg reactivity: mouse antibody type: monoclonal host species: armenian hamster formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , alexa fluor 488 anti mouse foxp3 antibody antibody clone: mf 14 isotype: rat igg2b, ? reactivity: mouse antibody type: monoclonal host species: rat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , ultra leaf purified anti human il 6 antibody antibody clone: mq2 13a5 isotype: rat igg1, ? reactivity: human antibody type: monoclonal host species: rat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , ultra leaf purified anti human tnf ? antibody antibody clone: mab1 isotype: mouse igg1, ? reactivity: human antibody type: monoclonal host species: mouse formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , ultra leaf purified anti human il 1? antibody antibody clone: h1b 27 isotype: mouse igg2b, ? reactivity: human antibody type: monoclonal host species: mouse formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial , true nuclear transcription factor buffer set buffer set contents: fix concentrate, nuclear fix diluent and perm buffer storage: 2°c 8°c. pack size: 1 pack of 120 tests , cyto fast fix / perm buffer set for fixation and permeabilization of mammalian cells for intracellular staining set contents: cyto fast fix / perm solution and cyto fast perm wash solution size: 1 pack of 300 tests storage: 2°c 8°c. , pe goat anti rat igg minimal x reactivity antibody antibody clone: poly4054 isotype: goat polyclonal igg reactivity: rat antibody type: polyclonal host species: goat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 200 ?g / vial , percp goat anti mouse igg minimal x reactivity antibody antibody clone: poly4053 isotype: goat polyclonal igg reactivity: mouse antibody type: polyclonal host species: goat formulation: phosphate buffered solution, ph 7.2, containing 0.09% sodium azide storage: 2°c 8°c size: 100 ?g / vial...

Directorate Of Purchase And Stores - Maharashtra

34547012 supply and safe delivery of fatty acids supply and safe delivery of fatty acids , supply and safe delivery of linoleic acid oleic acid albumin, 100x liquid, sterile filtered, bioreagent, suitable for cell culture biological source: protein from bovine form: liquid composition: linoleic acid and oleic acid, 2 mol / mol albumin ( each ) concentration: 100 mg / ml bsa in dpbs storage temp.: 2 8°c pack size: 5ml this product must be quoted in atleast 6 peer reviewed papers as a proof of authenticity. , lipid mixture 1, chemically defined liquid, sterile filtered, bioreagent, suitable for cell culture biological source: non animal source technique ( s ) : mammalian cell culture suitable impurities: =10 eu / ml endotoxin storage temp.: 2 8°c pack size: 100ml this product must be quoted in atleast 6 peer reviewed papers as a proof of authenticity. , oleic acid albumin from bovine serum liquid, sterile filtered, bioreagent, suitable for cell culture form: liquid composition: oleic acid, 2 mol / mol albumin concentration: 100 mg / ml bsa in dpbs technique ( s ) : mammalian cell culture suitable impurities: endotoxin, tested storage temp.: 2 8°c pack size: 5ml this product must be quoted in atleast 6 peer reviewed papers as a proof of authenticity. , linoleic acid albumin from bovine serum albumin liquid, sterile filtered, bioreagent, suitable for cell culture description: contains 2 moles linoleic acid per mole bsa form: liquid concentration: 100 mg / ml bsa in dpbs technique ( s ) : mammalian cell culture suitable impurities: =400 eu / ml endotoxin storage temp.: 2 8°c pack size: 5ml this product must be quoted in atleast 6 peer reviewed papers as a proof of authenticity. , ponceau s solution bioreagent, suitable for electrophoresis, 0.1 % ( w / v ) in 5% acetic acid form: liquid concentration: 0.1 % ( w / v ) in 5% acetic acid density: 1.01 g / cm3 at 20 °c applications: electrophoresis, diagnostic assay manufacturinghematology, histology storage temp.: room temp pack size: 1 l in poly bottle , sds sample buffer, laemmli 2× concentrate pack size: 1 pack of 10 vials storage temp: 20°c turbidity: clear color: blue form: liquid , ripa buffer description: protease free form: liquid packaging: pkg of 50 ml color: colorless ph: 7.8 8.2 storage temp.: 2 8°c , dimethyl sulfoxide hybri max grade, sterile filtered, bioreagent, suitable for hybridoma. suitability in cell freezing form: liquid pack size: 100 ml in amber bottle...

Directorate Of Purchase And Stores - Maharashtra

34129834 fetal bovine serum for cell culture fetal bovine serum for cell culture , fetal bovine serum for cell culture specifications: 1. the serum should be heat inactivated and usda approved is preferable. 2. the endotoxin level should be = 12 eu / ml. 3. the hemoglobin level should be = 50 mg / dl. 4. each bottle should be of 500 ml ( pack size: 1 bottle= 500 ml ) . 5. the species from which the serum is isolated should be cattle / bovine. 6. it should be shipped frozen @ 5 to 20 degrees centigrade on dry ice. 7. it should be compatible for mammalian cell culture. 8. it should be triple filtered at 0.1 ?m standard filter. 9. the bottle should be easy to use in the hood, should preferably reduce the risk of contamination. 10. there should be a peel off sticker on label to provide handy reference for lot number and expiration date recording in lab notebook. 11. it should be iso 13485 certified and processed in fda registered facilities. 12. should be virus and mycoplasma tested. should be supplied with a certificate of analysis detailing q.c results...

Food Corporation Of India - Maharashtra

32583715 supply of tissue culter flask, fetal calf serum, centrifuge tubes tissue culter supply of flask, fetal calf serum, dehydrated culture media centrifuge tubes. , tissue culture flask ( t 25 ) : box containing 200 numbers of tissue culture treated, non cytotoxic, non pyrogenic, sterile polystyrene flask with rectangular bottom, vented screw cap, canted neck, and graduations. cell growth area: 25 cm2, working volume: 5 7.5 ml , tissue culture flask ( t 75 ) : 1 box containing 100 numbers of tissue culture treated, non cytotoxic, non pyrogenic, sterile polystyrene flask with rectangular bottom, vented screw cap, canted neck, graduations, anti tip skirt and frosted marking area. cell growth area: 75 cm2, working volume:15 22.5 ml. , fetal calf serum: pack of 100 ml heat inactivated, filter sterilized fetal calf serum with <25mg / dl hemoglobin and suitable for mammalian cell culture. , yeast peptone dextrose broth, dehydrated culture media with 2 g / 100 ml dextrose, 500 g , 50 ml conical sterile polypropylene centrifuge tubes capacity ( metric ) : 50 ml, material: polypropylene, no. per case: 300, no. per pack: 25, sterility: sterile, shape: conical, type: centrifuge tube, max. force: 17, 000x g....

Department Of Biotechnology - Maharashtra

32437592 expression of interest for cum rfp for appointment of consultant for establishment of c gmp compliant mammalian cell line repository at nccs jidnyasa bldg, kothrud, pune...

Department Of Health And Family Welfare - Maharashtra

30476129 bids are invited for automated cell counter automated cell counter for counting mammalian cells in cell culture using one simple step with auto focus technology and disposable counting slides total quantity : 1...

Department Of Health And Family Welfare - Maharashtra

30294948 bids are invited for automated cell counter automated cell counter for counting mammalian cells in cell culture using one simple step with auto focus technology and disposable counting slides total quantity : 1...

Department Of Atomic Energy - Maharashtra

29458985 bids are invited for immun blot pvdf membrane , protease inhibitor cocktail for use with mammalian cell , precision plus protein dual color standards , protein assay dye reagent concentrate ,gsh/gssg assay kit, total quantity : 11...

Rashtriya Chemicals And Fertilizers Limited - Maharashtra

28521701 rate contract for carrying out toxicological studies from good laboratory practice ( glp ) certified laboratories of nano fertilizer developed by r and d ( bioagro ) department , rcf . human health safety ( env / jm / mono ( 2018 ) 4 ) : iata for skin corrosion and irritation ( env / jm / mono ( 2014 ) 19 ) 2 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 428 skin absorption: in vitro method 3 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 431 in vitro skin corrosion: reconstructed human epidermis ( rhe ) test method 4 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 437 bovine corneal opacity and permeability test method for evaluation of chemicals likely to cause serious eye damage 5 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 433 – acute inhalation toxicity for evaluation of health hazard from short term exposure to a test chemical by inhalation. 6 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd 436 acute inhalation toxicity: acute toxic class method 7 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 473 in vitro mammalian chromosomal aberration test 8 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 476 in vitro mammalian cell gene mutation tests using the hprt and xprt genes 9 human health safety ( env / jm / mono ( 2018 ) 4 ) :oecd tg 487 in vitro mammalian cell micronucleus test 10 human health safety ( env / jm / mono ( 2018 ) 4 ) :atp cell titer glo, neutral red uptake, ldh release, mtt, xtt, cell impedance, trypan blue, brdu, alamar blue, wst 1, live / dead cell counting, colony forming efficiency 11 human health safety ( env / jm / mono ( 2018 ) 4 ) : in addition, test no. 489 in vitro mammalian alkaline comet assay for measuring dna strand breaks in eukaryotic cells may also be included in the test panel. 12 environmental safety:oecd tg 201 aquatic test: fresh water algae, cyanobacteria and growth inhibition test 13 environmental safety:oecd tg 202 daphnia sp. acute immobilization test 14 environmental safety:oecd tg 203 fish acute toxicity test 15 environmental safety:oecd tg 211 daphnia magna reproduction test 16 environmental safety:oecd tg 236 fish embryo acute toxicity test on embryonic stages of fish ( daniorerio ) . 17 environmental safety:oecd tg 208 terrestrial plant test: seedling emergence and seedling growth test u.s. environmental protection agency ( us epa ) six bioassays: allium and vicia root tip chromosome breaks, tradescantia chromosome break, tradescantia micronucleus, tradescantia stamen hair mutation, and arabidopsis mutation bioassays for detecting the genotoxicity of environmental agents ( ma et al., 2005 ) . 18 effects on soil microbiota and macrobiota:oecd tg 222 – earthworm reproduction test for assessing the effects of chemicals in soil on the reproductive output ( and other sub lethal end points ) of the earthworm species eisenia fetida or eisenia andrei 19 effects on soil microbiota and macrobiota:oecd tg 216 for the long term effects after a single exposure of chemicals, on nitrogen transformation activity of soil microbiota 20 effects on soil microbiota and macrobiota:oecd 217 – to investigate long term effects after a single exposure of agrochemicals / nonagrochemicals on carbon transformation activity of soil microorganisms 21 effects on soil microbiota and macrobiota:oecd tg 471 bacterial reverse mutation test. additionally, clinical and laboratory standards institute, methods 02, 07, and 11 may be adopted for in vitro evaluation of antimicrobial susceptibility of naps and naips. suitable standard guidelines to evaluate risk to macrobiota may also be developed and provided by regulators. suitable evaluation method may be included to assess effect of naips on the alteration of biotic and abiotic soil properties. the outcome of toxicity tests may be used to establish thresholds or safe levels of chemicals in soil. methods for estimation of the half life of the nms in soil may also be included to predict ( potential ) risk. 22 effects on soil microbiota and macrobiota:oecd tg 307 may be adopted with necessary adaptations for aerobic anaerobic transformation of naips or naps in soil. 23 effects on soil microbiota and macrobiota:oecd guidelines for the testing of chemicals, section 3: test no. 318 may be adopted with modifications for naips and naps. 24 packaging compatibility data: decision framework ( oecd, env / jm / mono ( 2019 ) 12 ) for inclusion of physico chemical parameters for exposure and fate assessment of naips. 25 packaging compatibility data:avain dietary toxicity ( oecd205 ) 5 crop samples 26 packaging compatibility data:28 day repeated dose toxicity ( oecd407 ) 5crop samples 27 packaging compatibility data:leaching study ( oecd 312 ) 3 type soil and one plant 28 packaging compatibility data:nutritional analysis 5 crop samples...

Jawaharlal Nehru University - Maharashtra

8834986 we have optimised the adherence of mammalion cells using his all culture flask we use this flask routinely for long term cultivation and expansion of large number of cells with best protection of mammalian cells against contamination, cell culture flask t 75, with filter cap, tc treated sterile, non cytotoxic (0030 711.122) => short tender notice...

Directorate Of Purchase And Stores - Maharashtra

6105370 supply of coverslips (cell culture treated on one side for optimal mammalian cell growth and attachment, radiation sterilized) 0.2mm thickness, round, diameter 15mm make nunc or equivalent...